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Synthetic triacylated lipopeptide - TLR1/2
Synthetic triacylated lipopeptide
Pam3CSK4 is a synthetic triacylated lipopeptide (LP) that mimics the acylated amino terminus of bacterial LPs.
Pam3CSK4 is a potent activator of the proinflammatory transcription factor NF-κB.
Activation is mediated by the TLR2/TLR1 heterodimer, which recognizes LPs with three fatty acids, a structural characteristic of bacterial LPs.Back to the top
Specificity: TLR 1/2 agonist
Working concentration: 1 - 300 ng/ml
Molecular formula: C81H156N10O13S
Molecular weight: 1510.24
Solubility: 2 mg/ml in waterBack to the top
Pam3CSK4 is provided lyophilized.
- tlrl-pms: 1 mg Pam3CSK4 + 1.5 ml endotoxin-free water
Pam3CSK4 is shipped at room temperature.
Upon receipt it should be stored at 4°C.
Lyophilized Pam3CSK4 is stable 1 year when properly stored.
Upon resuspension, prepare aliquots of Pam3CSK4 and store at 4°C for short term storage or -20°C for long storage.
Resuspended product is stable 1 month at 4°C and 6 months at -20°C when properly stored.Back to the top
Bacterial lipoproteins are a family of proinflammatory cell wall components found in both Gram positive and Gram negative bacteria.
The stimulatory activity of bacterial lipoproteins resides in their acylated amino terminus. Pam3CSK4 is a synthetic tripalmitoylated lipopeptide that mimics the acylated amino terminus of bacterial lipoproteins.
Pam3CysSerLys4 (Pam3CSK4) is a potent activator of the proinflammatory transcription factor NF-κB .
Recognition of Pam3CSK4 is mediated by TLR2 which cooperates with TLR1 through their cytoplasmic domain to induce the signaling cascade leading to the activation of NF-κB .
1. Aliprantis AO et al., 1999. Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2. Science.285(5428):736-9.
2. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. PNAS. 97(25):13766-71.
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